5jue
From Proteopedia
(Difference between revisions)
m (Protected "5jue" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of UIC2 Fab== | |
| + | <StructureSection load='5jue' size='340' side='right' caption='[[5jue]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5jue]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JUE FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jue OCA], [http://pdbe.org/5jue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jue RCSB], [http://www.ebi.ac.uk/pdbsum/5jue PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jue ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | P-glycoprotein (P-gp) is a polyspecific ATP-dependent transporter linked to multidrug resistance in cancers that plays important roles in the pharmacokinetics of a large number of drugs. The drug-resistance phenotype of P-gp can be modulated by the monoclonal antibody UIC2, which specifically recognizes human P-gp in a conformation-dependent manner. Here, the purification, sequence determination and high-resolution structure of the Fab fragment of UIC2 (UIC2/Fab) are reported. Purified UIC2/Fab binds human P-gp with a 1:1 stoichiometry. Crystals of UIC2/Fab are triclinic (space group P1), with unit-cell parameters a = 40.67, b = 44.91, c = 58.09 A, alpha = 97.62, beta = 99.10, gamma = 94.09 degrees , and diffracted X-rays to 1.6 A resolution. The structure was determined by molecular replacement and refined to 1.65 A resolution. The asymmetric unit contains one molecule of UIC2/Fab, which exhibits a positively charged antigen-binding surface, suggesting that it might recognize an oppositely charged extracellular epitope of P-gp. | ||
| - | + | Crystal structure of the antigen-binding fragment of a monoclonal antibody specific for the multidrug-resistance-linked ABC transporter human P-glycoprotein.,Esser L, Shukla S, Zhou F, Ambudkar SV, Xia D Acta Crystallogr F Struct Biol Commun. 2016 Aug;72(Pt 8):636-41. doi:, 10.1107/S2053230X16009778. Epub 2016 Jul 27. PMID:27487928<ref>PMID:27487928</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5jue" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Esser, L]] | ||
| + | [[Category: Xia, D]] | ||
| + | [[Category: Antibody fab]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: P-glycoprotein]] | ||
| + | [[Category: Transport inhibition]] | ||
Revision as of 13:56, 10 September 2016
Crystal Structure of UIC2 Fab
| |||||||||||
