1isk

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[[Image:1isk.gif|left|200px]]
[[Image:1isk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1isk |SIZE=350|CAPTION= <scene name='initialview01'>1isk</scene>
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The line below this paragraph, containing "STRUCTURE_1isk", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=SIA:TYR+A14+And+ASP+A99+Both+Stabilize+The+Intermediate+By+F+...'>SIA</scene> and <scene name='pdbsite=SIB:TYR+B14+And+ASP+B99+Both+Stabilize+The+Intermediate+By+F+...'>SIB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1isk| PDB=1isk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1isk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1isk OCA], [http://www.ebi.ac.uk/pdbsum/1isk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1isk RCSB]</span>
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}}
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'''3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES'''
'''3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES'''
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[[Category: Zawrotny, M E.]]
[[Category: Zawrotny, M E.]]
[[Category: 3-ketosteroid]]
[[Category: 3-ketosteroid]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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[[Category: ksi]]
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[[Category: Ksi]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:21:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:24:02 2008''
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Revision as of 17:21, 2 May 2008

Image:1isk.gif

Template:STRUCTURE 1isk

3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES


Overview

The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.

About this Structure

1ISK is a Single protein structure of sequence from Comamonas testosteroni. Full crystallographic information is available from OCA.

Reference

Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200 Page seeded by OCA on Fri May 2 20:21:52 2008

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OCA

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