1j8l

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[[Image:1j8l.gif|left|200px]]
[[Image:1j8l.gif|left|200px]]
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{{Structure
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|PDB= 1j8l |SIZE=350|CAPTION= <scene name='initialview01'>1j8l</scene>, resolution 1.6&Aring;
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The line below this paragraph, containing "STRUCTURE_1j8l", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=C45:N4-METHOXY-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>C45</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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{{STRUCTURE_1j8l| PDB=1j8l | SCENE= }}
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|RELATEDENTRY=[[1i3t|1I3T]], [[1i47|1I47]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8l OCA], [http://www.ebi.ac.uk/pdbsum/1j8l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j8l RCSB]</span>
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'''Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA'''
'''Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA'''
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==About this Structure==
==About this Structure==
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1J8L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8L OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8L OCA].
==Reference==
==Reference==
Crystallographic studies on damaged DNAs IV. N( 4)-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis., Hossain MT, Sunami T, Tsunoda M, Hikima T, Chatake T, Ueno Y, Matsuda A, Takenaka A, Nucleic Acids Res. 2001 Oct 1;29(19):3949-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11574676 11574676]
Crystallographic studies on damaged DNAs IV. N( 4)-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis., Hossain MT, Sunami T, Tsunoda M, Hikima T, Chatake T, Ueno Y, Matsuda A, Takenaka A, Nucleic Acids Res. 2001 Oct 1;29(19):3949-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11574676 11574676]
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[[Category: Protein complex]]
 
[[Category: Chatake, T.]]
[[Category: Chatake, T.]]
[[Category: Hikima, T.]]
[[Category: Hikima, T.]]
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[[Category: Tsunoda, M.]]
[[Category: Tsunoda, M.]]
[[Category: Ueno, Y.]]
[[Category: Ueno, Y.]]
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[[Category: b-dna]]
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[[Category: B-dna]]
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[[Category: deoxyribonucleic acid]]
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[[Category: Deoxyribonucleic acid]]
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[[Category: dna damage]]
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[[Category: Dna damage]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: n4-methoxycytosine]]
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[[Category: N4-methoxycytosine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:55:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:30:05 2008''
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Revision as of 17:55, 2 May 2008

Image:1j8l.gif

Template:STRUCTURE 1j8l

Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA


Overview

To investigate the mutation mechanism of purine transitions in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCAAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and the crystal structure determined by X-ray analysis. The duplex structure is similar to that of the original undamaged B-form dodecamer, indicating that the methoxylation does not affect the overall DNA conformation. Electron density maps clearly show that the two mo(4)C residues form Watson-Crick-type base pairs with the adenine residues of the opposite strand and that the methoxy groups of mo(4)C adopt the anti conformation to N(3) around the C(4)-N(4) bond. For the pair formation through hydrogen bonds the mo(4)C residues are in the imino tautomeric state. Together with previous work, the present work establishes that the methoxylated cytosine residue can present two alternate faces for Watson-Crick base-pairing, thanks to the amino<-->imino tautomerism allowed by methoxylation. Based on this property, two gene transition routes are proposed.

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystallographic studies on damaged DNAs IV. N( 4)-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis., Hossain MT, Sunami T, Tsunoda M, Hikima T, Chatake T, Ueno Y, Matsuda A, Takenaka A, Nucleic Acids Res. 2001 Oct 1;29(19):3949-54. PMID:11574676 Page seeded by OCA on Fri May 2 20:55:16 2008

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