1j9y
From Proteopedia
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'''Crystal structure of mannanase 26A from Pseudomonas cellulosa''' | '''Crystal structure of mannanase 26A from Pseudomonas cellulosa''' | ||
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[[Category: Woo, E J.]] | [[Category: Woo, E J.]] | ||
[[Category: 4/7-superfamily of glycoside hydrolase]] | [[Category: 4/7-superfamily of glycoside hydrolase]] | ||
| - | [[Category: | + | [[Category: Beta/alpha barrel]] |
| - | [[Category: | + | [[Category: Clan gh-a]] |
| - | [[Category: | + | [[Category: Family 26 glycoside hydrolase]] |
| - | [[Category: | + | [[Category: Tim barrel]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:57:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:57, 2 May 2008
Crystal structure of mannanase 26A from Pseudomonas cellulosa
Overview
The crystal structure of Pseudomonas cellulosa mannanase 26A has been solved by multiple isomorphous replacement and refined at 1.85 A resolution to an R-factor of 0.182 (R-free = 0.211). The enzyme comprises (beta/alpha)(8)-barrel architecture with two catalytic glutamates at the ends of beta-strands 4 and 7 in precisely the same location as the corresponding glutamates in other 4/7-superfamily glycoside hydrolase enzymes (clan GH-A glycoside hydrolases). The family 26 glycoside hydrolases are therefore members of clan GH-A. Functional analyses of mannanase 26A, informed by the crystal structure of the enzyme, provided important insights into the role of residues close to the catalytic glutamates. These data showed that Trp-360 played a critical role in binding substrate at the -1 subsite, whereas Tyr-285 was important to the function of the nucleophile catalyst. His-211 in mannanase 26A does not have the same function as the equivalent asparagine in the other GH-A enzymes. The data also suggest that Trp-217 and Trp-162 are important for the activity of mannanase 26A against mannooligosaccharides but are less important for activity against polysaccharides.
About this Structure
1J9Y is a Single protein structure of sequence from Cellvibrio japonicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding., Hogg D, Woo EJ, Bolam DN, McKie VA, Gilbert HJ, Pickersgill RW, J Biol Chem. 2001 Aug 17;276(33):31186-92. Epub 2001 May 29. PMID:11382747 Page seeded by OCA on Fri May 2 20:57:41 2008
