1jef

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[[Image:1jef.gif|left|200px]]
[[Image:1jef.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1jef |SIZE=350|CAPTION= <scene name='initialview01'>1jef</scene>, resolution 1.77&Aring;
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The line below this paragraph, containing "STRUCTURE_1jef", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1jef| PDB=1jef | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jef OCA], [http://www.ebi.ac.uk/pdbsum/1jef PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jef RCSB]</span>
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}}
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'''TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3'''
'''TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3'''
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[[Category: Harata, K.]]
[[Category: Harata, K.]]
[[Category: Muraki, M.]]
[[Category: Muraki, M.]]
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[[Category: bacteriolytic enzyme]]
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[[Category: Bacteriolytic enzyme]]
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[[Category: enzyme]]
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[[Category: Enzyme]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: inhibitor complex]]
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[[Category: Inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:07:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:32:29 2008''
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Revision as of 18:07, 2 May 2008

Image:1jef.gif

Template:STRUCTURE 1jef

TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3


Overview

The turkey-egg lysozyme (TEL) complex with tri-N-acetylchitotriose [(GlcNac)3] was co-crystallized from 1.5% TEL and 2 mM (GlcNac)3 at pH 4.2. The crystal structure was determined by molecular replacement and refined to an R value of 0.182 using 10-1.77 A data. The (GlcNac)3 molecule occupies the subsites A, B and C. At the subsites B and C, the sugar residues are bound in a similar manner to that found in the hen-egg lysozyme (HEL) complex. In contrast, the GlcNac residue at the subsite A is exposed to bulk solvent and has no contact with the protein molecule because the active residue Asp101 in HEL is replaced by Gly in TEL. A sulfate ion is bound in the vicinity of subsite B and forms hydrogen bonds with the sugar residue and the guanidino group of Arg61, assisting the binding of the sugar residue to subsite B. The active-site cleft of TEL is narrower than that of native TEL, thus attaining the best fit of the (GlcNac)3 molecule. The lack of binding ability of subsite A is discussed in relation to the catalytic properties of TEL. The result suggests that the cleavage pattern of oligosaccharide substrates in the catalytic reaction is regulated by the protein-sugar interaction at subsite A.

About this Structure

1JEF is a Single protein structure of sequence from Meleagris gallopavo. Full crystallographic information is available from OCA.

Reference

X-ray structure of turkey-egg lysozyme complex with tri-N-acetylchitotriose. Lack of binding ability at subsite A., Harata K, Muraki M, Acta Crystallogr D Biol Crystallogr. 1997 Nov 1;53(Pt 6):650-7. PMID:15299852 Page seeded by OCA on Fri May 2 21:07:22 2008

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OCA

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