1jeq
From Proteopedia
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'''Crystal Structure of the Ku Heterodimer''' | '''Crystal Structure of the Ku Heterodimer''' | ||
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[[Category: Goldberg, J.]] | [[Category: Goldberg, J.]] | ||
[[Category: Walker, J R.]] | [[Category: Walker, J R.]] | ||
| - | [[Category: | + | [[Category: Alpha/beta domain]] |
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Double-strand dna break repair]] |
| - | [[Category: | + | [[Category: Helical c-terminal arm]] |
| - | [[Category: | + | [[Category: Non-homologous end-joining]] |
| - | [[Category: | + | [[Category: Sap domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:07:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:07, 2 May 2008
Crystal Structure of the Ku Heterodimer
Overview
The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle two full turns of DNA while encircling only the central 3-4 base pairs (bp). Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation.
About this Structure
1JEQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair., Walker JR, Corpina RA, Goldberg J, Nature. 2001 Aug 9;412(6847):607-14. PMID:11493912 Page seeded by OCA on Fri May 2 21:07:55 2008
