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1jhi
From Proteopedia
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[[Image:1jhi.gif|left|200px]] | [[Image:1jhi.gif|left|200px]] | ||
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'''Solution Structure of a Hedamycin-DNA complex''' | '''Solution Structure of a Hedamycin-DNA complex''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHI OCA]. | |
==Reference== | ==Reference== | ||
Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11820806 11820806] | Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11820806 11820806] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Burley, G A.]] | [[Category: Burley, G A.]] | ||
[[Category: Carver, J A.]] | [[Category: Carver, J A.]] | ||
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[[Category: Owen, E A.]] | [[Category: Owen, E A.]] | ||
[[Category: Wickham, G.]] | [[Category: Wickham, G.]] | ||
| - | [[Category: | + | [[Category: Duplex dna]] |
| - | [[Category: | + | [[Category: Hedamycin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:13:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:13, 2 May 2008
Solution Structure of a Hedamycin-DNA complex
Overview
Hedamycin, a member of the pluramycin family of drugs, displays a range of biological responses including antitumor and antimicrobial activity. The mechanism of action is via direct interaction with DNA through intercalation between the bases of the oligonucleotide and alkylation of a guanine residue at 5'-PyG-3' sites. There appears to be some minor structural differences between two earlier studies on the interaction of hedamycin with 5'-PyG-3' sites. In this study, a high-resolution NMR analysis of the hedamycin:d(ACCGGT)2 complex was undertaken in order to investigate the effect of replacing the thymine with a guanine at the preferred 5'-CGT-3' site. The resultant structure was compared with earlier work, with particular emphasis placed on the drug conformation. The structure of the hedamycin:d(ACCGGT)2 complex has many features in common with the two previous NMR structures of hedamycin:DNA complexes but differed in the conformation and orientation of the N,N-dimethylvancosamine saccharide of hedamycin in one of these structures. The preferential binding of hedamycin to 5'-CG-3' over 5'-TG-3' binding sites is explained in terms of the orientation and location of the N,N-dimethylvancosamine saccharide in the minor groove.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:11820806 Page seeded by OCA on Fri May 2 21:13:45 2008
