1jhh
From Proteopedia
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'''LEXA S119A MUTANT''' | '''LEXA S119A MUTANT''' | ||
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[[Category: Pfuetzner, R A.]] | [[Category: Pfuetzner, R A.]] | ||
[[Category: Strynadka, N C.J.]] | [[Category: Strynadka, N C.J.]] | ||
| - | [[Category: | + | [[Category: Lexa sos repressor]] |
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Revision as of 18:13, 2 May 2008
LEXA S119A MUTANT
Overview
LexA repressor undergoes a self-cleavage reaction. In vivo, this reaction requires an activated form of RecA, but it occurs spontaneously in vitro at high pH. Accordingly, LexA must both allow self-cleavage and yet prevent this reaction in the absence of a stimulus. We have solved the crystal structures of several mutant forms of LexA. Strikingly, two distinct conformations are observed, one compatible with cleavage, and the other in which the cleavage site is approximately 20 A from the catalytic center. Our analysis provides insight into the structural and energetic features that modulate the interconversion between these two forms and hence the rate of the self-cleavage reaction. We suggest RecA activates the self-cleavage of LexA and related proteins through selective stabilization of the cleavable conformation.
About this Structure
1JHH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of LexA: a conformational switch for regulation of self-cleavage., Luo Y, Pfuetzner RA, Mosimann S, Paetzel M, Frey EA, Cherney M, Kim B, Little JW, Strynadka NC, Cell. 2001 Sep 7;106(5):585-94. PMID:11551506 Page seeded by OCA on Fri May 2 21:13:43 2008
