1jiy

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[[Image:1jiy.jpg|left|200px]]
[[Image:1jiy.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1jiy |SIZE=350|CAPTION= <scene name='initialview01'>1jiy</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1jiy", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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{{STRUCTURE_1jiy| PDB=1jiy | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jiy OCA], [http://www.ebi.ac.uk/pdbsum/1jiy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jiy RCSB]</span>
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'''CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 20% SORBITOL'''
'''CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 20% SORBITOL'''
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[[Category: Datta, S.]]
[[Category: Datta, S.]]
[[Category: Vijayan, M.]]
[[Category: Vijayan, M.]]
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[[Category: enzyme-tetragonal form]]
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[[Category: Enzyme-tetragonal form]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: hen egg-white lysozyme]]
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[[Category: Hen egg-white lysozyme]]
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[[Category: mucopeptide n-acetylmuramyl hydrolase]]
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[[Category: Mucopeptide n-acetylmuramyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:16:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:34:25 2008''
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Revision as of 18:16, 2 May 2008

Image:1jiy.jpg

Template:STRUCTURE 1jiy

CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 20% SORBITOL


Overview

In order to elucidate the effect of stabilizing additives on the structure of proteins and the associated ordered water molecules in the hydration shell, the crystal structures of tetragonal lysozyme grown in the presence of sucrose, sorbitol and trehalose have been refined. Also refined are the structures of orthorhombic and monoclinic lysozyme grown under the conditions in which tetragonal lysozyme is normally grown. A comparison of the two sets of structures with the structure of native tetragonal lysozyme shows that the effect of the additives on the structure of the protein molecule is less than that of the normal minor changes associated with differences in molecular packing. Surprisingly, the same is true of the effect on the hydration shell, represented by the ordered water molecules attached to the protein. Thus, it appears that the cause of the stabilizing effect of the additives needs to be sought outside the immediate neighbourhood of the protein molecule. Sorbitol and trehalose do not coherently interact with the protein. One sucrose molecule binds at the active-site cleft of the enzyme.

About this Structure

1JIY is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

The effect of stabilizing additives on the structure and hydration of proteins: a study involving tetragonal lysozyme., Datta S, Biswal BK, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1614-20. Epub 2001, Oct 25. PMID:11679726 Page seeded by OCA on Fri May 2 21:16:54 2008

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