1jjx
From Proteopedia
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[[Image:1jjx.jpg|left|200px]] | [[Image:1jjx.jpg|left|200px]] | ||
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'''Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein''' | '''Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein''' | ||
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[[Category: Zimmerman, A W.]] | [[Category: Zimmerman, A W.]] | ||
[[Category: 15n isotope enrichment]] | [[Category: 15n isotope enrichment]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Fatty acid carrier]] |
| - | [[Category: | + | [[Category: Lipid binding protein]] |
| - | [[Category: | + | [[Category: Nmr spectroscopy]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:18:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:18, 2 May 2008
Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein
Overview
Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.
About this Structure
1JJX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of fatty acid-binding protein from human brain., Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C, Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569 Page seeded by OCA on Fri May 2 21:18:49 2008
