1jl0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1jl0.jpg|left|200px]]
[[Image:1jl0.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1jl0 |SIZE=350|CAPTION= <scene name='initialview01'>1jl0</scene>, resolution 1.50&Aring;
+
The line below this paragraph, containing "STRUCTURE_1jl0", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosylmethionine_decarboxylase Adenosylmethionine decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.50 4.1.1.50] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1jl0| PDB=1jl0 | SCENE= }}
-
|RELATEDENTRY=[[1jen|1JEN]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl0 OCA], [http://www.ebi.ac.uk/pdbsum/1jl0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jl0 RCSB]</span>
+
-
}}
+
'''Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant'''
'''Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant'''
Line 31: Line 28:
[[Category: Tolbert, W D.]]
[[Category: Tolbert, W D.]]
[[Category: Xiong, H.]]
[[Category: Xiong, H.]]
-
[[Category: allosteric enzyme]]
+
[[Category: Allosteric enzyme]]
-
[[Category: decarboxylase]]
+
[[Category: Decarboxylase]]
-
[[Category: ester intermediate]]
+
[[Category: Ester intermediate]]
-
[[Category: hydroxyalanine]]
+
[[Category: Hydroxyalanine]]
-
[[Category: lyase]]
+
[[Category: Lyase]]
-
[[Category: pyruvate]]
+
[[Category: Pyruvate]]
-
[[Category: pyruvoyl]]
+
[[Category: Pyruvoyl]]
-
[[Category: s-adenosylmethionine]]
+
[[Category: S-adenosylmethionine]]
-
[[Category: sandwich]]
+
[[Category: Sandwich]]
-
[[Category: spermidine biosynthesis]]
+
[[Category: Spermidine biosynthesis]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:21:01 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:35:11 2008''
+

Revision as of 18:21, 2 May 2008

Image:1jl0.jpg

Template:STRUCTURE 1jl0

Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant


Overview

S-Adenosylmethionine decarboxylase (AdoMetDC) is synthesized as a proenzyme that cleaves itself in a putrescine-stimulated reaction via an N-->O acyl shift and beta-elimination to produce an active enzyme with a catalytically essential pyruvoyl residue at the new N-terminus. N-->O acyl shifts initiate the self-processing of other proteins such as inteins and amidohydrolases, but their mechanisms in such proteins are not well understood. We have solved the crystal structure of the H243A mutant of AdoMetDC to 1.5 A resolution. The mutant protein is trapped in the ester form, providing clear evidence for the structure of the ester intermediate in the processing of pyruvoyl enzymes. In addition, a putrescine molecule is bound in a charged region within the beta-sandwich, and cross-links the two beta-sheets through hydrogen bonds to several acidic residues and ordered water molecules. The high-resolution structure provides insight into the mechanism for the self-processing reaction and provides evidence for the mechanism for simulation of the self-processing reaction by putrescine. Studies of the effects of putrescine or 4-aminobutanol on the processing of mutant AdoMetDC proenzymes are consistent with a model in which a single activator molecule interacts with buried Asp174, Glu178, and Glu256, leading to an alteration in the position of Glu11, resulting in stimulation of self-processing.

About this Structure

1JL0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a human S-adenosylmethionine decarboxylase self-processing ester intermediate and mechanism of putrescine stimulation of processing as revealed by the H243A mutant., Ekstrom JL, Tolbert WD, Xiong H, Pegg AE, Ealick SE, Biochemistry. 2001 Aug 14;40(32):9495-504. PMID:11583148 Page seeded by OCA on Fri May 2 21:21:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jl0"
Personal tools