1jlk
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1jlk.gif|left|200px]] | [[Image:1jlk.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1jlk", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1jlk| PDB=1jlk | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Crystal structure of the Mn(2+)-bound form of response regulator Rcp1''' | '''Crystal structure of the Mn(2+)-bound form of response regulator Rcp1''' | ||
| Line 33: | Line 30: | ||
[[Category: Song, P S.]] | [[Category: Song, P S.]] | ||
[[Category: Yang, S S.]] | [[Category: Yang, S S.]] | ||
| - | [[Category: | + | [[Category: Cph1]] |
| - | [[Category: | + | [[Category: Cyanobacterial phytochrome]] |
| - | [[Category: | + | [[Category: Rcp1]] |
| - | [[Category: | + | [[Category: Response regulator]] |
| - | [[Category: | + | [[Category: Two-component system]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:22:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:22, 2 May 2008
Crystal structure of the Mn(2+)-bound form of response regulator Rcp1
Overview
The two-component signal transduction pathway widespread in prokaryotes, fungi, molds, and some plants involves an elaborate phosphorelay cascade. Rcp1 is the phosphate receiver module in a two-component system controlling the light response of cyanobacteria Synechocystis sp. via cyanobacterial phytochrome Cph1, which recognizes Rcp1 and transfers its phosphoryl group to an aspartate residue in response to light. Here we describe the crystal structure of Rcp1 refined to a crystallographic R-factor of 18.8% at a resolution of 1.9 A. The structure reveals a tightly associated homodimer with monomers comprised of doubly wound five-stranded parallel beta-sheets forming a single-domain protein homologous with the N-terminal activator domain of other response regulators (e.g., chemotaxis protein CheY). The three-dimensional structure of Rcp1 appears consistent with the conserved activation mechanism of phosphate receiver proteins, although in this case, the C-terminal half of its regulatory domain, which undergoes structural changes upon phosphorylation, contributes to the dimerization interface. The involvement of the residues undergoing phosphorylation-induced conformational changes at the dimeric interface suggests that dimerization of Rcp1 may be regulated by phosphorylation, which could affect the interaction of Rcp1 with downstream target molecules.
About this Structure
1JLK is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of a cyanobacterial phytochrome response regulator., Im YJ, Rho SH, Park CM, Yang SS, Kang JG, Lee JY, Song PS, Eom SH, Protein Sci. 2002 Mar;11(3):614-24. PMID:11847283 Page seeded by OCA on Fri May 2 21:22:24 2008
