1jms

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[[Image:1jms.jpg|left|200px]]
[[Image:1jms.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1jms |SIZE=350|CAPTION= <scene name='initialview01'>1jms</scene>, resolution 2.36&Aring;
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The line below this paragraph, containing "STRUCTURE_1jms", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_nucleotidylexotransferase DNA nucleotidylexotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.31 2.7.7.31] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TDT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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-->
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|DOMAIN=
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{{STRUCTURE_1jms| PDB=1jms | SCENE= }}
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|RELATEDENTRY=[[1bpx|1bpx]], [[1bpy|1bpy]], [[1bpz|1bpz]], [[1kan|1kan]], [[1kny|1kny]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jms OCA], [http://www.ebi.ac.uk/pdbsum/1jms PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jms RCSB]</span>
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}}
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'''Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase'''
'''Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase'''
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[[Category: Rougeon, F.]]
[[Category: Rougeon, F.]]
[[Category: Sukumar, N.]]
[[Category: Sukumar, N.]]
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[[Category: nucleotidyl transferase]]
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[[Category: Nucleotidyl transferase]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:25:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:35:57 2008''
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Revision as of 18:25, 2 May 2008

Image:1jms.jpg

Template:STRUCTURE 1jms

Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase


Overview

The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 A resolution reveals a typical DNA polymerase beta-like fold locked in a closed form. In addition, the structures of two different binary complexes, one with an oligonucleotide primer and the other with an incoming ddATP-Co(2+) complex, show that the substrates and the two divalent ions in the catalytic site are positioned in TdT in a manner similar to that described for the human DNA polymerase beta ternary complex, suggesting a common two metal ions mechanism of nucleotidyl transfer in these two proteins. The inability of TdT to accommodate a template strand can be explained by steric hindrance at the catalytic site caused by a long lariat-like loop, which is absent in DNA polymerase beta. However, displacement of this discriminating loop would be sufficient to unmask a number of evolutionarily conserved residues, which could then interact with a template DNA strand. The present structure can be used to model the recently discovered human polymerase mu, with which it shares 43% sequence identity.

About this Structure

1JMS is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase., Delarue M, Boule JB, Lescar J, Expert-Bezancon N, Jourdan N, Sukumar N, Rougeon F, Papanicolaou C, EMBO J. 2002 Feb 1;21(3):427-39. PMID:11823435 Page seeded by OCA on Fri May 2 21:25:25 2008

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