1jou
From Proteopedia
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'''Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site''' | '''Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site''' | ||
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[[Category: Esmon, C T.]] | [[Category: Esmon, C T.]] | ||
[[Category: Huntington, J A.]] | [[Category: Huntington, J A.]] | ||
| - | [[Category: | + | [[Category: Enzyme]] |
| - | [[Category: | + | [[Category: Factor iia]] |
| - | [[Category: | + | [[Category: Protease]] |
| - | [[Category: | + | [[Category: Proteinase]] |
| - | [[Category: | + | [[Category: Thrombin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:31:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:31, 2 May 2008
Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site
Overview
Thrombin participates in its own positive and negative feedback loops, and its allosteric state helps determine the hemostatic balance. Here we present the 1.8 A crystallographic structure of S195A thrombin in two conformational states: active site occupied and active site free. The active site-occupied form shows how thrombin can accommodate substrates, such as protein C. The active site-free form is in a previously unobserved closed conformation of thrombin, which satisfies all the conditions of the so-called "slow" form. A mechanism of allostery is revealed, which relies on the concerted movement of the disulphide bond between Cys168 and 182 and aromatic residues Phe227, Trp215, and Trp60d. These residues constitute an allosteric switch, which is flipped directly through sodium binding, resulting in the fast form with an open active site.
About this Structure
1JOU is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The molecular basis of thrombin allostery revealed by a 1.8 A structure of the "slow" form., Huntington JA, Esmon CT, Structure. 2003 Apr;11(4):469-79. PMID:12679024 Page seeded by OCA on Fri May 2 21:31:36 2008
