5f8i
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8i OCA], [http://pdbe.org/5f8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f8i RCSB], [http://www.ebi.ac.uk/pdbsum/5f8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f8i ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8i OCA], [http://pdbe.org/5f8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f8i RCSB], [http://www.ebi.ac.uk/pdbsum/5f8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f8i ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Viral RNA-dependent RNA polymerases (RdRPs) play essential roles in viral genome replication and transcription. We previously reported several structural states of the poliovirus RdRP nucleotide addition cycle (NAC) that revealed a unique palm domain-based active site closure mechanism and proposed a six-state NAC model including a hypothetical state representing translocation intermediates. Using the RdRP from another human enterovirus, enterovirus 71, here we report seven RdRP elongation complex structures derived from a crystal lattice that allows three NAC events. These structures suggested a key order of events in initial NTP binding and NTP-induced active site closure and revealed a bona fide translocation intermediate featuring asymmetric movement of the template-product duplex. Our work provides essential missing links in understanding NTP recognition and translocation mechanisms in viral RdRPs and emphasizes the uniqueness of the viral RdRPs compared with other processive polymerases. | ||
| + | |||
| + | Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation.,Shu B, Gong P Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):E4005-14. doi:, 10.1073/pnas.1602591113. Epub 2016 Jun 23. PMID:27339134<ref>PMID:27339134</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5f8i" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 20:42, 3 August 2016
Enterovirus 71 Polymerase Elongation Complex (C1S2/3 Form)
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