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3oa7

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Revision as of 07:19, 12 May 2022

Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae

Structural highlights

3oa7 is a 1 chain structure with sequence from Bpph2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	7, CNM67, YNL225C, N1264 (BPPH2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The spindle pole body of the budding yeast Saccharomyces cerevisiae has served as a model system for understanding microtubule organizing centers, yet very little is known about the molecular structure of its components. We report here the structure of the C-terminal domain of the core component Cnm67 at 2.3 A resolution. The structure determination was aided by a novel approach to crystallization of proteins containing coiled-coils that utilizes globular domains to stabilize the coiled-coils. This enhances their solubility in Escherichia coli and improves their crystallization. The Cnm67 C-terminal domain (residues Asn-429-Lys-581) exhibits a previously unseen dimeric, interdigitated, all alpha-helical fold. In vivo studies demonstrate that this domain alone is able to localize to the spindle pole body. In addition, the structure reveals a large functionally indispensable positively charged surface patch that is implicated in spindle pole body localization. Finally, the C-terminal eight residues are disordered but are critical for protein folding and structural stability.

Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body.,Klenchin VA, Frye JJ, Jones MH, Winey M, Rayment I J Biol Chem. 2011 May 20;286(20):18240-50. Epub 2011 Mar 24. PMID:21454609^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Klenchin VA, Frye JJ, Jones MH, Winey M, Rayment I. Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body. J Biol Chem. 2011 May 20;286(20):18240-50. Epub 2011 Mar 24. PMID:21454609 doi:http://dx.doi.org/10.1074/jbc.M111.227371

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3oa7"

@@ Line 1: / Line 1: @@
 ==Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae==
-<StructureSection load='3oa7' size='340' side='right' caption='[[3oa7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='3oa7' size='340' side='right'caption='[[3oa7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3oa7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph2 Bpph2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OA7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3oa7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpph2 Bpph2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OA7 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7, CNM67, YNL225C, N1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 BPPH2])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7, CNM67, YNL225C, N1264 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 BPPH2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oa7 OCA], [http://pdbe.org/3oa7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3oa7 RCSB], [http://www.ebi.ac.uk/pdbsum/3oa7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3oa7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oa7 OCA], [https://pdbe.org/3oa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oa7 RCSB], [https://www.ebi.ac.uk/pdbsum/3oa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oa7 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 22: / Line 22: @@
 </StructureSection>
 [[Category: Bpph2]]
+[[Category: Large Structures]]
 [[Category: Frye, J J]]
 [[Category: Klenchin, V A]]

3oa7

From Proteopedia

Revision as of 07:19, 12 May 2022

Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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