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5iqt
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqt OCA], [http://pdbe.org/5iqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iqt RCSB], [http://www.ebi.ac.uk/pdbsum/5iqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqt ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqt OCA], [http://pdbe.org/5iqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iqt RCSB], [http://www.ebi.ac.uk/pdbsum/5iqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A 2.4-A-resolution X-ray crystal structure of the carrier-protein-independent halogenase WelO5 in complex with its welwitindolinone precursor substrate, 12-epi-fischerindole U, reveals that the C13 chlorination target is proximal to the anticipated site of the oxo group in a presumptive cis-halo-oxo-iron(IV) (haloferryl) intermediate. Prior study of related halogenases forecasts substrate hydroxylation in this active-site configuration, but X-ray crystallographic verification of C13 halogenation in single crystals mandates that ligand dynamics must reposition the oxygen ligand to enable the observed outcome. S189A WelO5 produces a mixture of halogenation and hydroxylation products, showing that an outer-sphere hydrogen-bonding group orchestrates ligand movements to achieve a configuration that promotes halogen transfer. | ||
| + | |||
| + | Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5.,Mitchell AJ, Zhu Q, Maggiolo AO, Ananth NR, Hillwig ML, Liu X, Boal AK Nat Chem Biol. 2016 Jun 27. doi: 10.1038/nchembio.2112. PMID:27348090<ref>PMID:27348090</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5iqt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 10:25, 13 July 2016
WelO5 bound to Fe(II), Cl, 2-oxoglutarate, and 12-epifischerindole U
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