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1jqs
From Proteopedia
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[[Image:1jqs.gif|left|200px]] | [[Image:1jqs.gif|left|200px]] | ||
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'''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog''' | '''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog''' | ||
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[[Category: Segupta, J.]] | [[Category: Segupta, J.]] | ||
[[Category: 70s e coli ribosome]] | [[Category: 70s e coli ribosome]] | ||
| - | [[Category: | + | [[Category: Cryo-em]] |
| - | [[Category: | + | [[Category: Ef-g]] |
| - | [[Category: | + | [[Category: Gtp state]] |
| - | [[Category: | + | [[Category: L11]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:41:04 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:41, 2 May 2008
Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog
Overview
L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.
About this Structure
1JQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530 Page seeded by OCA on Fri May 2 21:41:04 2008
Categories: Escherichia coli | Protein complex | Agrawal, R K. | Frank, J. | Linde, J. | Nierhaus, K H. | Segupta, J. | 70s e coli ribosome | Cryo-em | Ef-g | Gtp state | L11
