This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5jj8
From Proteopedia
(Difference between revisions)
| Line 10: | Line 10: | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/A0A072ZBL6_PSEAI A0A072ZBL6_PSEAI]] Reversible hydration of carbon dioxide.[RuleBase:RU003956] | [[http://www.uniprot.org/uniprot/A0A072ZBL6_PSEAI A0A072ZBL6_PSEAI]] Reversible hydration of carbon dioxide.[RuleBase:RU003956] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cryoannealing has been demonstrated to improve the diffraction quality and resolution of crystals of the beta-carbonic anhydrase psCA3 concomitant with a change in space group. After initial flash-cooling in a liquid-nitrogen cryostream an X-ray diffraction data set from a psCA3 crystal was indexed in space group P21212 and was scaled to 2.6 A resolution, but subsequent cryoannealing studies revealed induced protein rearrangements in the crystal contacts, which transformed the space group to I222, with a corresponding improvement of 0.7 A in resolution. Although the change in diffraction resolution was significant, only minor changes in the psCA3 structure, which retained its catalytic `open' conformation, were observed. These findings demonstrate that cryoannealing can be successfully utilized to induce higher diffraction-quality crystals while maintaining enzymatically relevant conformations and may be useful as an experimental tool for structural studies of other enzymes where the initial diffraction quality is poor. | ||
| + | |||
| + | Cryoannealing-induced space-group transition of crystals of the carbonic anhydrase psCA3.,Pinard MA, Kurian JJ, Aggarwal M, Agbandje-McKenna M, McKenna R Acta Crystallogr F Struct Biol Commun. 2016 Jul 1;72(Pt 7):573-7. doi:, 10.1107/S2053230X16009286. Epub 2016 Jun 28. PMID:27380376<ref>PMID:27380376</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5jj8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:43, 26 July 2016
Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa - alternate crystal packing form
| |||||||||||
