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1jvz

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[[Image:1jvz.gif|left|200px]]
[[Image:1jvz.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1jvz", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=CEN:7BETA-(4CARBOXYBUTANAMIDO)+CEPHALOSPORANIC+ACID'>CEN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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|RELATEDENTRY=[[1jw0|1JW0]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jvz OCA], [http://www.ebi.ac.uk/pdbsum/1jvz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jvz RCSB]</span>
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'''Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid'''
'''Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid'''
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[[Category: Hol, W G.J.]]
[[Category: Hol, W G.J.]]
[[Category: Kim, Y.]]
[[Category: Kim, Y.]]
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[[Category: cephalosporin acylase]]
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[[Category: Cephalosporin acylase]]
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[[Category: glutaryl-7-aminocephalosporanic acid]]
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[[Category: Glutaryl-7-aminocephalosporanic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:50 2008''
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Revision as of 18:59, 2 May 2008

Image:1jvz.gif

Template:STRUCTURE 1jvz

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid


Overview

BACKGROUND: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC. RESULTS: The binary complex structures of CA with GL-7-ACA and glutarate (the side-chain of GL-7-ACA) show extensive interactions between the glutaryl moiety of GL-7-ACA and the seven residues that form the side-chain pocket. These interactions explain why the D-alpha-aminoadipyl side-chain of CPC yields a poorer substrate than GL-7-ACA. CONCLUSIONS: This understanding of the nature of substrate specificity may be useful in the design of an enzyme with an improved performance for the conversion of CPC to 7-ACA. Additionally, the catalytic mechanism of the deacylation reaction was revealed by the ligand bound structures.

About this Structure

1JVZ is a Protein complex structure of sequences from Brevundimonas diminuta. Full crystallographic information is available from OCA.

Reference

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity., Kim Y, Hol WG, Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403 Page seeded by OCA on Fri May 2 21:59:37 2008

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