1jxg
From Proteopedia
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'''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge''' | '''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge''' | ||
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[[Category: Milani, M.]] | [[Category: Milani, M.]] | ||
[[Category: Verbeet, M P.]] | [[Category: Verbeet, M P.]] | ||
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Revision as of 19:02, 2 May 2008
The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge
Overview
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
About this Structure
1JXG is a Single protein structure of sequence from Populus nigra. Full crystallographic information is available from OCA.
Reference
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761 Page seeded by OCA on Fri May 2 22:02:29 2008
