This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1k19
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1k19.jpg|left|200px]] | [[Image:1k19.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1k19", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1k19| PDB=1k19 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae''' | '''NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae''' | ||
| Line 31: | Line 28: | ||
[[Category: Mosbah, A.]] | [[Category: Mosbah, A.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
| - | [[Category: | + | [[Category: Chemosensory]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Pheromone]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:10:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:10, 2 May 2008
NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae
Overview
Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. A number of such proteins, of molecular mass approximately 13 kDa, have been isolated from different sensory organs of a wide range of insect species. Several CSPs have been identified in the antennae and proboscis of the moth Mamestra brassicae. CSPMbraA6, a 112-amino-acid antennal protein, has been expressed in a soluble form in large quantities in the Escherichi coli periplasm. NMR structure determination of CSPMbraA6 has been performed with 1H- and 15N-labelled samples. The calculated structures present an average root mean square deviation about the mean structure of 0.63 A for backbone atoms and 1.27 A for all non-hydrogen atoms except the 12 N-terminal residues. The protein is well folded from residue 12 to residue 110, and consists of a non-bundle alpha-helical structure with six helices connected by alpha alpha loops. It has a globular shape, with overall dimensions of 32 A x 28 A x 24 A. A channel is visible in the hydrophobic core, with dimensions of 3 A x 9 A x 21 A. In some of the 20 solution structures calculated, this channel is closed either by Trp-94 at one end or by Tyr-26 at the other end; in some other solutions, this channel is closed at both ends. Binding experiments with 12-bromododecanol indicate that the CSPMbraA6 structure is modified upon ligand binding.
About this Structure
1K19 is a Single protein structure of sequence from Mamestra brassicae. Full crystallographic information is available from OCA.
Reference
Solution structure of a chemosensory protein from the moth Mamestra brassicae., Mosbah A, Campanacci V, Lartigue A, Tegoni M, Cambillau C, Darbon H, Biochem J. 2003 Jan 1;369(Pt 1):39-44. PMID:12217077 Page seeded by OCA on Fri May 2 22:10:22 2008
