1a2w
From Proteopedia
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| - | [[Image:1a2w. | + | [[Image:1a2w.jpg|left|200px]]<br /><applet load="1a2w" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1a2w" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1a2w, resolution 2.10Å" /> | caption="1a2w, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A'''<br /> | '''CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A2W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CL and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | + | 1A2W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CL and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=AVE:The Active Site Contains Residues From Different Subunits'>AVE</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A2W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:07:47 2007'' |
Revision as of 11:58, 18 December 2007
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CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A
Overview
The dimer of bovine pancreatic ribonuclease A (RNase A) discovered by, Crestfield, Stein, and Moore in 1962 has been crystallized and its, structure determined and refined to a 2.1-A resolution. The dimer is 3D, domain-swapped. The N-terminal helix (residues 1-15) of each subunit is, swapped into the major domain (residues 23-124) of the other subunit. The, dimer of bull seminal ribonuclease (BS-RNase) is also known to be, domain-swapped, but the relationship of the subunits within the two dimers, is strikingly different. In the RNase A dimer, the 3-stranded beta sheets, of the two subunits are hydrogen-bonded at their edges to form a, continuous 6-stranded sheet across the dimer interface; in the BS-RNase, dimer, it is instead the two helices that abut. Whereas the BS-RNase dimer, has 2-fold molecular symmetry, the two subunits of the RNase A dimer are, related by a rotation of approximately 160 degrees. Taken together, these, structures show that intersubunit adhesion comes mainly from the swapped, helical domain binding to the other subunit in the "closed interface" but, that the overall architecture of the domain-swapped oligomer depends on, the interactions in the second type of interface, the "open interface.", The RNase A dimer crystals take up the dye Congo Red, but the structure of, a Congo Red-stained crystal reveals no bound dye molecule. Dimer formation, is inhibited by excess amounts of the swapped helical domain. The possible, implications for amyloid formation are discussed.
About this Structure
1A2W is a Single protein structure of sequence from Bos taurus with CL and SO4 as ligands. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution., Liu Y, Hart PJ, Schlunegger MP, Eisenberg D, Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3437-42. PMID:9520384
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