1k2g
From Proteopedia
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'''Structural basis for the 3'-terminal guanosine recognition by the group I intron''' | '''Structural basis for the 3'-terminal guanosine recognition by the group I intron''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2G OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron., Kitamura A, Muto Y, Watanabe S, Kim I, Ito T, Nishiya Y, Sakamoto K, Ohtsuki T, Kawai G, Watanabe K, Hosono K, Takaku H, Katoh E, Yamazaki T, Inoue T, Yokoyama S, RNA. 2002 Apr;8(4):440-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11991639 11991639] | Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron., Kitamura A, Muto Y, Watanabe S, Kim I, Ito T, Nishiya Y, Sakamoto K, Ohtsuki T, Kawai G, Watanabe K, Hosono K, Takaku H, Katoh E, Yamazaki T, Inoue T, Yokoyama S, RNA. 2002 Apr;8(4):440-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11991639 11991639] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hosono, K.]] | [[Category: Hosono, K.]] | ||
[[Category: Inoue, T.]] | [[Category: Inoue, T.]] | ||
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[[Category: Yamazaki, T.]] | [[Category: Yamazaki, T.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| - | [[Category: | + | [[Category: Base triple recognition of a guanosine]] |
| - | [[Category: | + | [[Category: Gaga tetraloop]] |
| - | [[Category: | + | [[Category: Uucg tetraloop]] |
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Revision as of 19:13, 2 May 2008
Structural basis for the 3'-terminal guanosine recognition by the group I intron
Overview
In the second step of the two consecutive transesterifications of the self-splicing reaction of the group I intron, the conserved guanosine at the 3' terminus of the intron (omegaG) binds to the guanosine-binding site (GBS) in the intron. In the present study, we designed a 22-nt model RNA (GBS/omegaG) including the GBS and omegaG from the Tetrahymena group I intron, and determined the solution structure by NMR methods. In this structure, omegaG is recognized by the formation of a base triple with the G264 x C311 base pair, and this recognition is stabilized by the stacking interaction between omegaG and C262. The bulged structure at A263 causes a large helical twist angle (40 +/- 80) between the G264 x C311 and C262 x G312 base pairs. We named this type of binding pocket with a bulge and a large twist, formed on the major groove, a "Bulge-and-Twist" (BT) pocket. With another twist angle between the C262 x G312 and G413 x C313 base pairs (45 +/- 100), the axis of GBS/omegaG is kinked at the GBS region. This kinked axis superimposes well on that of the corresponding region in the structure model built on a 5.0 A resolution electron density map (Golden et al., Science, 1998, 282:345-358). This compact structure of the GBS is also consistent with previous biochemical studies on group I introns. The BT pockets are also found in the arginine-binding site of the HIV-TAR RNA, and within the 16S rRNA and the 23S rRNA.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron., Kitamura A, Muto Y, Watanabe S, Kim I, Ito T, Nishiya Y, Sakamoto K, Ohtsuki T, Kawai G, Watanabe K, Hosono K, Takaku H, Katoh E, Yamazaki T, Inoue T, Yokoyama S, RNA. 2002 Apr;8(4):440-51. PMID:11991639 Page seeded by OCA on Fri May 2 22:13:19 2008
