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1k3f

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[[Image:1k3f.jpg|left|200px]]
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{{Structure
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|PDB= 1k3f |SIZE=350|CAPTION= <scene name='initialview01'>1k3f</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1k3f", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] </span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3f OCA], [http://www.ebi.ac.uk/pdbsum/1k3f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k3f RCSB]</span>
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'''Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice'''
'''Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice'''
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[[Category: Smirnova, E A.]]
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[[Category: Vainshtein, B K.]]
[[Category: Vainshtein, B K.]]
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[[Category: hexamer]]
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[[Category: Hexamer]]
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[[Category: nucleoside phosphorylase]]
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[[Category: Nucleoside phosphorylase]]
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Revision as of 19:15, 2 May 2008

Template:STRUCTURE 1k3f

Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice


Overview

Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments.

About this Structure

1K3F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice., Morgunova EYu, Mikhailov AM, Popov AN, Blagova EV, Smirnova EA, Vainshtein BK, Mao C, Armstrong ShR, Ealick SE, Komissarov AA, et al., FEBS Lett. 1995 Jun 26;367(2):183-7. PMID:7796917 Page seeded by OCA on Fri May 2 22:15:09 2008

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