1k4u
From Proteopedia
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'''Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox''' | '''Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox''' | ||
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[[Category: Sumimoto, H.]] | [[Category: Sumimoto, H.]] | ||
[[Category: Takeya, R.]] | [[Category: Takeya, R.]] | ||
| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | [[Category: | + | [[Category: P47phox]] |
| - | [[Category: | + | [[Category: P67phox]] |
| - | [[Category: | + | [[Category: Sh3-peptide complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:18:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:18, 2 May 2008
Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox
Contents |
Overview
The basic function of the Src homology 3 (SH3) domain is considered to be binding to proline-rich sequences containing a PxxP motif. Recently, many SH3 domains, including those from Grb2 and Pex13p, were reported to bind sequences lacking a PxxP motif. We report here that the 22 residue peptide lacking a PxxP motif, derived from p47(phox), binds to the C-terminal SH3 domain from p67(phox). We applied the NMR cross-saturation method to locate the interaction sites for the non-PxxP peptides on their cognate SH3 domains from p67(phox), Grb2 and Pex13p. The binding site of the Grb2 SH3 partially overlapped the conventional PxxP-binding site, whereas those of p67(phox) and Pex13p SH3s are located in different surface regions. The non-PxxP peptide from p47(phox) binds to the p67(phox) SH3 more tightly when it extends to the N-terminus to include a typical PxxP motif, which enabled the structure determination of the complex, to reveal that the non-PxxP peptide segment interacted with the p67(phox) SH3 in a compact helix-turn-helix structure (PDB entry 1K4U).
Disease
Known disease associated with this structure: Chronic granulomatous disease due to deficiency of NCF-2 OMIM:[608515], Chronic granulomatous disease due to deficiency of NCF-1 OMIM:[608512]
About this Structure
1K4U is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p., Kami K, Takeya R, Sumimoto H, Kohda D, EMBO J. 2002 Aug 15;21(16):4268-76. PMID:12169629 Page seeded by OCA on Fri May 2 22:18:33 2008
