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1k6g
From Proteopedia
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[[Image:1k6g.gif|left|200px]] | [[Image:1k6g.gif|left|200px]] | ||
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'''Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing''' | '''Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6G OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743001 11743001] | Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743001 11743001] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Elela, S Abou.]] | [[Category: Elela, S Abou.]] | ||
[[Category: Fourmy, D.]] | [[Category: Fourmy, D.]] | ||
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[[Category: Lebars, I.]] | [[Category: Lebars, I.]] | ||
[[Category: Yoshizawa, S.]] | [[Category: Yoshizawa, S.]] | ||
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Revision as of 19:21, 2 May 2008
Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing
Overview
Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:11743001 Page seeded by OCA on Fri May 2 22:21:53 2008
