5ks8
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus== | |
| + | <StructureSection load='5ks8' size='340' side='right' caption='[[5ks8]], [[Resolution|resolution]] 3.01Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ks8]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KS8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ks8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks8 OCA], [http://pdbe.org/5ks8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ks8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ks8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pyruvate carboxylase (PC) has important roles in metabolism and is crucial for virulence for some pathogenic bacteria. PC contains biotin carboxylase (BC), carboxyltransferase (CT) and biotin carboxyl carrier protein (BCCP) components. It is a single-chain enzyme in eukaryotes and most bacteria, and functions as a 500 kD homo-tetramer. In contrast, PC is a two-subunit enzyme in a collection of Gram-negative bacteria, with the alpha subunit containing the BC and the beta subunit the CT and BCCP domains, and it is believed that the holoenzyme has alpha4beta4 stoichiometry. We report here the crystal structures of a two-subunit PC from Methylobacillus flagellatus. Surprisingly, our structures reveal an alpha2beta4 stoichiometry, and the overall architecture of the holoenzyme is strikingly different from that of the homo-tetrameric PCs. Biochemical and mutagenesis studies confirm the stoichiometry and other structural observations. Our functional studies in Pseudomonas aeruginosa show that its two-subunit PC is important for colony morphogenesis. | ||
| - | + | A distinct holoenzyme organization for two-subunit pyruvate carboxylase.,Choi PH, Jo J, Lin YC, Lin MH, Chou CY, Dietrich LE, Tong L Nat Commun. 2016 Oct 6;7:12713. doi: 10.1038/ncomms12713. PMID:27708276<ref>PMID:27708276</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5ks8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Choi, P H]] | ||
[[Category: Tong, L]] | [[Category: Tong, L]] | ||
| - | [[Category: | + | [[Category: Biotin]] |
| + | [[Category: Ligase]] | ||
| + | [[Category: Pyruvate]] | ||
| + | [[Category: Tim barrel]] | ||
Revision as of 17:22, 19 October 2016
Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus
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Categories: Choi, P H | Tong, L | Biotin | Ligase | Pyruvate | Tim barrel
