1ka8
From Proteopedia
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'''Crystal Structure of the Phage P4 Origin-Binding Domain''' | '''Crystal Structure of the Phage P4 Origin-Binding Domain''' | ||
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[[Category: Yeo, H J.]] | [[Category: Yeo, H J.]] | ||
[[Category: Ziegelin, G.]] | [[Category: Ziegelin, G.]] | ||
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Revision as of 19:29, 2 May 2008
Crystal Structure of the Phage P4 Origin-Binding Domain
Overview
The origin-binding domain of the gpalpha protein of phage P4 (P4-OBD) mediates origin recognition and regulation of gpalpha activity by the protein Cnr. We have determined the crystal structure of P4-OBD at 2.95 A resolution. The structure of P4-OBD is that of a dimer with pseudo twofold symmetry. Each subunit has a winged helix topology with a unique structure among initiator proteins. The only structural homologue of the P4-OBD subunit is the DNA-binding domain of the eukaryotic transcriptional activator Rfx1. Based on this structural alignment, a model for origin recognition by the P4-OBD dimer is suggested. P4-OBD mutations that interfere with Cnr binding locate to the dimer interface, indicating that Cnr acts by disrupting the gpalpha dimer. P4-OBD dimerization is mediated by helices alpha1 and alpha3 in both subunits, a mode of winged helix protein dimerization that is reminiscent of that of the eukaryotic transcription factors E2F and DP. This, in turn, suggests that Cnr is also a winged helix protein, a possibility that is supported by previously unreported sequence homologies between Cnr and Rfx1 and homology modelling. Hence, in a mechanism that appears to be conserved from phage to man, the DNA-binding activity of winged helix proteins can be regulated by other winged helix proteins via the versatile use of the winged helix motif as a homo- or heterodimerization scaffold.
About this Structure
1KA8 is a Single protein structure of sequence from Enterobacteria phage p4. Full crystallographic information is available from OCA.
Reference
Phage P4 origin-binding domain structure reveals a mechanism for regulation of DNA-binding activity by homo- and heterodimerization of winged helix proteins., Yeo HJ, Ziegelin G, Korolev S, Calendar R, Lanka E, Waksman G, Mol Microbiol. 2002 Feb;43(4):855-67. PMID:11929537 Page seeded by OCA on Fri May 2 22:29:47 2008
