1kb1
From Proteopedia
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[[Image:1kb1.gif|left|200px]] | [[Image:1kb1.gif|left|200px]] | ||
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'''SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE CYTOSINE''' | '''SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE CYTOSINE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KB1 OCA]. | |
==Reference== | ==Reference== | ||
Structural effect of the anticancer agent 6-thioguanine on duplex DNA., Bohon J, de los Santos CR, Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12582253 12582253] | Structural effect of the anticancer agent 6-thioguanine on duplex DNA., Bohon J, de los Santos CR, Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12582253 12582253] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Bohon, J.]] | [[Category: Bohon, J.]] | ||
[[Category: Santos, C R.De Los.]] | [[Category: Santos, C R.De Los.]] | ||
[[Category: 6-thioguanine]] | [[Category: 6-thioguanine]] | ||
[[Category: 6tg]] | [[Category: 6tg]] | ||
| - | [[Category: | + | [[Category: B-form dna]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | [[Category: | + | [[Category: S6g]] |
| - | [[Category: | + | [[Category: Tg]] |
| - | [[Category: | + | [[Category: Thioguanine]] |
| - | [[Category: | + | [[Category: Thiopurine]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:31:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:31, 2 May 2008
SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE CYTOSINE
Overview
The incorporation of 6-thioguanine (S6G) into DNA is an essential step in the cytotoxic activity of thiopurines. However, the structural effects of this substitution on duplex DNA have not been fully characterized. Here, we present the solution structures of DNA duplexes containing S6G opposite thymine (S6G.T) and opposite cytosine (S6G.C), solved by high-resolution NMR spectroscopy and restrained molecular dynamics. The data indicate that both duplexes adopt right-handed helical conformations with all Watson-Crick hydrogen bonding in place. The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymine shifted toward the major groove and S6G displaced toward the minor groove. Aside from the lesion site, the helices, including the flanking base pairs, are not highly perturbed by the presence of the lesion. Surprisingly, thermal dependence experiments suggest greater stability in the S6G-T mismatch than the S6G-C base pair.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural effect of the anticancer agent 6-thioguanine on duplex DNA., Bohon J, de los Santos CR, Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:12582253 Page seeded by OCA on Fri May 2 22:31:23 2008
Categories: Bohon, J. | Santos, C R.De Los. | 6-thioguanine | 6tg | B-form dna | Double helix | S6g | Tg | Thioguanine | Thiopurine
