Purine repressor
From Proteopedia
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| - | <StructureSection load=' | + | <StructureSection load='1wet' size='350' side='right' caption='Structure of E. coli purine processor complex with DNA (green) and guanine (PDB entry [[1wet]])' scene=''> |
== Function == | == Function == | ||
| - | '''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. | + | '''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. |
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| + | == Structural highlights == | ||
| + | The PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones. The DNA binding domain contains a helix-turn-helix-loop-helix motif which interacts with the DNA major groove and a hinge helix binding to to the DNA minor groove. Residue L53 interdigitates with the DNA central base pair<ref>PMID:9278422</ref>. | ||
</StructureSection> | </StructureSection> | ||
Revision as of 10:00, 31 July 2016
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3D structures of purine repressor
Updated on 31-July-2016
References
- ↑ Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621
- ↑ Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422
