1kcb
From Proteopedia
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'''Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction''' | '''Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction''' | ||
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Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction., Liu SQ, Chang T, Liu MY, LeGall J, Chang WC, Zhang JP, Liang DC, Chang WR, Biochem Biophys Res Commun. 2003 Mar 14;302(3):568-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12615072 12615072] | Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction., Liu SQ, Chang T, Liu MY, LeGall J, Chang WC, Zhang JP, Liang DC, Chang WR, Biochem Biophys Res Commun. 2003 Mar 14;302(3):568-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12615072 12615072] | ||
[[Category: Achromobacter cycloclastes]] | [[Category: Achromobacter cycloclastes]] | ||
| - | [[Category: Nitrite reductase (NO-forming)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chang, T.]] | [[Category: Chang, T.]] | ||
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[[Category: Liu, S Q.]] | [[Category: Liu, S Q.]] | ||
[[Category: Zhang, J P.]] | [[Category: Zhang, J P.]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Copper-containing nitrite reductase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:34:03 2008'' | |
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Revision as of 19:34, 2 May 2008
Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction
Overview
I257E was obtained by site directed mutagenesis of nitrite reductase from Achromobacter cycloclastes. The mutant has no enzyme activity. Its crystal structure determined at 1.65A resolution shows that the side-chain carboxyl group of the mutated residue, Glu257, coordinates with the type 2 copper in the mutant and blocks the contact between the type 2 copper and its solvent channel, indicating that the accessibility of the type 2 copper is essential for maintaining the activity of nitrite reductase. The carboxylate is an analog of the substrate, nitrite, but the distances between the type 2 copper and the two oxygen atoms of the side-chain carboxyl group are reversed in comparison to the binding of nitrite to the native enzyme. In the mutant, both the type 2 copper and the N epsilon atom on the imidazole ring of its coordinated residue His135 move in the substrate binding direction relative to the native enzyme. In addition, an EPR study showed that the type 2 copper in the mutant is in a reduced state. We propose that mutant I257E is in a state corresponding to a transition state in the enzymatic reaction.
About this Structure
1KCB is a Single protein structure of sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA.
Reference
Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction., Liu SQ, Chang T, Liu MY, LeGall J, Chang WC, Zhang JP, Liang DC, Chang WR, Biochem Biophys Res Commun. 2003 Mar 14;302(3):568-74. PMID:12615072 Page seeded by OCA on Fri May 2 22:34:03 2008
