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1kf3
From Proteopedia
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'''Atomic Resolution Structure of RNase A at pH 5.9''' | '''Atomic Resolution Structure of RNase A at pH 5.9''' | ||
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[[Category: Wilson, K S.]] | [[Category: Wilson, K S.]] | ||
[[Category: Zagari, A.]] | [[Category: Zagari, A.]] | ||
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| - | [[Category: | + | [[Category: Rnase some]] |
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| - | [[Category: | + | [[Category: Titration]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:39:57 2008'' | |
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Revision as of 19:39, 2 May 2008
Atomic Resolution Structure of RNase A at pH 5.9
Overview
The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
About this Structure
1KF3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829 Page seeded by OCA on Fri May 2 22:39:57 2008
Categories: Bos taurus | Pancreatic ribonuclease | Single protein | Berisio, R. | Lamzin, V S. | Mazzarella, L. | Sica, F. | Wilson, K S. | Zagari, A. | Crystal | Ph | Rnase some | Soaking | Titration
