1kfa
From Proteopedia
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'''Crystal structure of Fab fragment complexed with gibberellin A4''' | '''Crystal structure of Fab fragment complexed with gibberellin A4''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFA OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054627 12054627] | Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054627 12054627] | ||
| - | [[Category: Homo sapiens]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Asami, O.]] | [[Category: Asami, O.]] | ||
[[Category: Fushinobu, S.]] | [[Category: Fushinobu, S.]] | ||
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[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
| - | [[Category: | + | [[Category: Immunoglobuiln fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:40, 2 May 2008
Crystal structure of Fab fragment complexed with gibberellin A4
Overview
Gibberellins, a class of plant hormones, consist of more than 120 members. Only a few of them are recognized by a receptor that remains unknown. The haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody and GA(4). We carried out an X-ray crystallographic analysis of the 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by using the molecular replacement method. The crystal structure of the Fab fragment showed the typical immunoglobulin fold of the beta-barrel structure which is the common motif of all antibodies. A small hapten-combining site was made up of three heavy chain CDR loops. On the other hand, CDRs of the light chain did not interact directly with GA(4). The C/D rings of the GA(4) molecule were in van der Waals contact mainly with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3 beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:12054627 Page seeded by OCA on Fri May 2 22:40:30 2008
