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4jmo

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Revision as of 11:23, 24 November 2022

Complexe of ARNO Sec7 domain with the protein-protein interaction inhibitor N-(4-hydroxy-2,6-dimethylphenyl)-4-methoxybenzenesulfonamide

Structural highlights

4jmo is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYH2_HUMAN Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.^[1]

Publication Abstract from PubMed

By virtual screening using a Fragment-Based Drug Design (FBDD) approach, 33 fragments were selected within small pockets around interaction hot spots on the Sec7 surface of the nucleotide exchange factor Arno, and then their ability to interfere with the Arno-catalyzed nucleotide exchange on the G-protein Arf1 was evaluated. Using SPR, NMR and fluorescence assays, the direct binding of three of the identified fragments to Arno Sec7 domain was demonstrated and the promiscuous aggregate behavior evaluated. Then, the binding mode of one fragment and of a more active analogue was solved by X-ray crystallography. This highlighted the role of stable and transient pockets at the Sec7 domain surface in the discovery and binding of interfering compounds. These results provide structural information on how small organic compounds can interfere with the Arf1-Arno Sec7 domain interaction and may guide the rational drug design of competitive inhibitors of Arno enzymatic activity.

Fragment-Based Identification of a Locus in the Sec7 Domain of Arno for the Design of Protein-Protein Interaction Inhibitors.,Jad R, Hoh F, Estaran S, Henriquet C, Boublik Y, Kerkour A, Trouillard R, Martinez J, Pugniere M, Padilla A, Chavanieu A J Med Chem. 2013 Oct 11. PMID:24112024^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hofmann I, Thompson A, Sanderson CM, Munro S. The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane. Curr Biol. 2007 Apr 17;17(8):711-6. Epub 2007 Mar 29. PMID:17398095 doi:http://dx.doi.org/10.1016/j.cub.2007.03.007
↑ Jad R, Hoh F, Estaran S, Henriquet C, Boublik Y, Kerkour A, Trouillard R, Martinez J, Pugniere M, Padilla A, Chavanieu A. Fragment-Based Identification of a Locus in the Sec7 Domain of Arno for the Design of Protein-Protein Interaction Inhibitors. J Med Chem. 2013 Oct 11. PMID:24112024 doi:http://dx.doi.org/10.1021/jm4009357

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4jmo"

Categories: Homo sapiens | Large Structures | Hoh F | Rouhana J

@@ Line 1: / Line 1: @@
 ==Complexe of ARNO Sec7 domain with the protein-protein interaction inhibitor N-(4-hydroxy-2,6-dimethylphenyl)-4-methoxybenzenesulfonamide==
-<StructureSection load='4jmo' size='340' side='right' caption='[[4jmo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='4jmo' size='340' side='right'caption='[[4jmo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4jmo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JMO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4jmo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JMO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JAF:N-(4-HYDROXY-2,6-DIMETHYLPHENYL)-4-METHOXYBENZENESULFONAMIDE'>JAF</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JAF:N-(4-HYDROXY-2,6-DIMETHYLPHENYL)-4-METHOXYBENZENESULFONAMIDE'>JAF</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jmi|4jmi]], [[4jwl|4jwl]], [[4jxh|4jxh]], [[4l5m|4l5m]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jmo OCA], [https://pdbe.org/4jmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jmo RCSB], [https://www.ebi.ac.uk/pdbsum/4jmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jmo ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYTH2, ARNO, PSCD2, PSCD2L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jmo OCA], [http://pdbe.org/4jmo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jmo RCSB], [http://www.ebi.ac.uk/pdbsum/4jmo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jmo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CYH2_HUMAN CYH2_HUMAN]] Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.<ref>PMID:17398095</ref>
+[https://www.uniprot.org/uniprot/CYH2_HUMAN CYH2_HUMAN] Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.<ref>PMID:17398095</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Hoh, F]]
+[[Category: Large Structures]]
-[[Category: Rouhana, J]]
+[[Category: Hoh F]]
-[[Category: Arf1]]
+[[Category: Rouhana J]]
-[[Category: Nucleotide exchange factor]]
-[[Category: Signaling protein-inhibitor complex]]

4jmo

From Proteopedia

Revision as of 11:23, 24 November 2022

Complexe of ARNO Sec7 domain with the protein-protein interaction inhibitor N-(4-hydroxy-2,6-dimethylphenyl)-4-methoxybenzenesulfonamide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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