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1kl9
From Proteopedia
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[[Image:1kl9.jpg|left|200px]] | [[Image:1kl9.jpg|left|200px]] | ||
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'''Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha''' | '''Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha''' | ||
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[[Category: Nonato, M C.]] | [[Category: Nonato, M C.]] | ||
[[Category: Widom, J.]] | [[Category: Widom, J.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:52:31 2008'' | |
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Revision as of 19:52, 2 May 2008
Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha
Overview
Eukaryotic translation initiation factor 2alpha (eIF2alpha) is a member of the eIF2 heterotrimeric complex that binds and delivers Met-tRNA(i)(Met) to the 40 S ribosomal subunit in a GTP-dependent manner. Phosphorylation/dephosphorylation of eIF2alpha at Ser-51 is the major regulator of protein synthesis in eukaryotic cells. Here, we report the first structural analysis on eIF2, the three-dimensional structure of a 22-kDa N-terminal portion of human eIF2alpha by x-ray diffraction at 1.9 A resolution. This structure contains two major domains. The N terminus is a beta-barrel with five antiparallel beta-strands in an oligonucleotide binding domain (OB domain) fold. The phosphorylation site (Ser-51) is on the loop connecting beta3 and beta4 in the OB domain. A helical domain follows the OB domain, and the first helix has extensive interactions, including a disulfide bridge, to fix its orientation with respect to the OB domain. The two domains meet along a negatively charged groove with highly conserved residues, indicating a likely site for protein-protein interaction.
About this Structure
1KL9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the N-terminal segment of human eukaryotic translation initiation factor 2alpha., Nonato MC, Widom J, Clardy J, J Biol Chem. 2002 May 10;277(19):17057-61. Epub 2002 Feb 21. PMID:11859078 Page seeded by OCA on Fri May 2 22:52:31 2008
