1ae7
From Proteopedia
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| - | [[Image:1ae7.gif|left|200px]]<br /> | + | [[Image:1ae7.gif|left|200px]]<br /><applet load="1ae7" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ae7" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1ae7, resolution 2.0Å" /> | caption="1ae7, resolution 2.0Å" /> | ||
'''NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2'''<br /> | '''NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AE7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | + | 1AE7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Known structural/functional Sites: <scene name='pdbsite=CAL:Site *Cal* Comprises Those Residues Forming The Ca2+ Bin ...'>CAL</scene> and <scene name='pdbsite=CNR:Site *Cnr* Comprises Those Residues Forming The Catalyti ...'>CNR</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AE7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: venom]] | [[Category: venom]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:11:48 2007'' |
Revision as of 12:02, 18 December 2007
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NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2
Overview
The three-dimensional structure of notexin has been solved by molecular, replacement methods. The structure has been refined at 2.0 A resolution to, a crystallographic R-value of 16.5% with good stereo-chemistry. The core, of the protein is very similar to other phospholipase A2s (PLA2 s) but, several parts of the molecule are distinctly different. The most, significant differences from PLA2 s from bovine pancreas and rattlesnake, occur in the stretches 56-80 and 85-89. Residue 69, which has been shown, to be important for phospholipase binding, has a different conformation, and different interactions than in other known PLA2s. The C alpha, positions for residues 86-88 differ by about 6 A from both the bovine and, the rattlesnake enzyme. The crystals contain no Ca2+ ions. Instead, a, water molecule occupies the calcium site.
About this Structure
1AE7 is a Single protein structure of sequence from Notechis scutatus scutatus with SO4 as ligand. Active as Phospholipase A(2), with EC number 3.1.1.4 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0 A resolution., Westerlund B, Nordlund P, Uhlin U, Eaker D, Eklund H, FEBS Lett. 1992 Apr 20;301(2):159-64. PMID:1568473
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