1kpq
From Proteopedia
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'''Structure of the Tsg101 UEV domain''' | '''Structure of the Tsg101 UEV domain''' | ||
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[[Category: Rich, R L.]] | [[Category: Rich, R L.]] | ||
[[Category: Sundquist, W I.]] | [[Category: Sundquist, W I.]] | ||
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Revision as of 20:01, 2 May 2008
Structure of the Tsg101 UEV domain
Overview
Human Tsg101 plays key roles in HIV budding and in cellular vacuolar protein sorting (VPS). In performing these functions, Tsg101 binds both ubiquitin (Ub) and the PTAP tetrapeptide 'late domain' motif located within the viral Gag protein. These interactions are mediated by the N-terminal domain of Tsg101, which belongs to the catalytically inactive ubiquitin E2 variant (UEV) family. We now report the structure of Tsg101 UEV and chemical shift mapping of the Ub and PTAP binding sites. Tsg101 UEV resembles canonical E2 ubiquitin conjugating enzymes, but has an additional N-terminal helix, an extended beta-hairpin that links strands 1 and 2, and lacks the two C-terminal helices normally found in E2 enzymes. PTAP-containing peptides bind in a hydrophobic cleft exposed by the absence of the C-terminal helices, whereas ubiquitin binds in a novel site surrounding the beta-hairpin. These studies provide a structural framework for understanding how Tsg101 mediates the protein-protein interactions required for HIV budding and VPS.
About this Structure
1KPQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and functional interactions of the Tsg101 UEV domain., Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI, EMBO J. 2002 May 15;21(10):2397-406. PMID:12006492 Page seeded by OCA on Fri May 2 23:01:23 2008
