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1kq3

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[[Image:1kq3.gif|left|200px]]
[[Image:1kq3.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1kq3 |SIZE=350|CAPTION= <scene name='initialview01'>1kq3</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1kq3", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|GENE= TM0423 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK09423 gldA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1454 EutG]</span>
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{{STRUCTURE_1kq3| PDB=1kq3 | SCENE= }}
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|RELATEDENTRY=[[1jpu|1JPU]], [[1jq5|1JQ5]], [[1jqa|1JQA]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq3 OCA], [http://www.ebi.ac.uk/pdbsum/1kq3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kq3 RCSB]</span>
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}}
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'''Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution'''
'''Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution'''
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[[Category: Miller, M D.]]
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[[Category: Wilson, I A]]
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[[Category: glycerol dehydrogenase]]
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[[Category: Glycerol dehydrogenase]]
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[[Category: jcsg]]
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[[Category: Jcsg]]
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[[Category: joint center for structural genomic]]
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[[Category: Joint center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:02:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:59 2008''
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Revision as of 20:02, 2 May 2008

Image:1kq3.gif

Template:STRUCTURE 1kq3

Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution


Overview

Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

About this Structure

1KQ3 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline., Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Epub 2002 Aug 22. PMID:12193646 Page seeded by OCA on Fri May 2 23:02:02 2008

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