4o8b

Publication Abstract from PubMed

Pseudomonas aeruginosa relies on cell motility and ability to form biofilms to establish infections; however, the mechanism of regulation remains obscure. Here we report that BswR, a xenobiotic response element-type transcriptional regulator, plays a critical role in regulation of bacterial motility and biofilm formation in P. aeruginosa. Transcriptomic and biochemical analyses showed that BswR counteracts the repressor activity of MvaT, controls the transcription of small RNA rsmZ and regulates the biogenesis of bacterial flagella. The crystal structure of BswR was determined at 2.3 A resolution; the monomer comprises a DNA-binding domain with a helix-turn-helix motif in the N terminus and two helices (alpha6 and alpha7) with a V-shaped arrangement in the C-terminus. In addition to the contacts between the parallel helices alpha5 of two monomers, the two helical extensions (alpha6 and alpha7) intertwine together to form a homodimer, which is the biological function unit. Based on the result of DNase I protection assay together with structural analysis of BswR homodimer, we proposed a BswR-DNA model, which suggests a molecular mechanism with which BswR could interact with DNA. Taken together, our results unveiled a novel regulatory mechanism, in which BswR controls the motility and biofilm formation of P. aeruginosa by modulating the transcription of small RNA rsmZ.

BswR controls bacterial motility and biofilm formation in Pseudomonas aeruginosa through modulation of the small RNA rsmZ.,Wang C, Ye F, Kumar V, Gao YG, Zhang LH Nucleic Acids Res. 2014 Feb 3. PMID:24497189^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.