1ksw

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[[Image:1ksw.gif|left|200px]]
[[Image:1ksw.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ksw |SIZE=350|CAPTION= <scene name='initialview01'>1ksw</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1ksw", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NBS:N6-BENZYL+ADENOSINE-5&#39;-DIPHOSPHATE'>NBS</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ksw| PDB=1ksw | SCENE= }}
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|RELATEDENTRY=[[1fmk|1FMK]], [[2src|2SRC]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ksw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksw OCA], [http://www.ebi.ac.uk/pdbsum/1ksw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ksw RCSB]</span>
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}}
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'''Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP'''
'''Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP'''
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[[Category: Shokat, K M.]]
[[Category: Shokat, K M.]]
[[Category: Witucki, L A.]]
[[Category: Witucki, L A.]]
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[[Category: atp]]
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[[Category: Atp]]
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[[Category: bump hole]]
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[[Category: Bump hole]]
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[[Category: bump-hole]]
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[[Category: Bump-hole]]
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[[Category: chemical genetic]]
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[[Category: Chemical genetic]]
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[[Category: kinase]]
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[[Category: Kinase]]
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[[Category: orthogonal substrate]]
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[[Category: Orthogonal substrate]]
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[[Category: sh2]]
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[[Category: Sh2]]
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[[Category: sh3]]
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[[Category: Sh3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:08:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:53:07 2008''
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Revision as of 20:08, 2 May 2008

Image:1ksw.gif

Template:STRUCTURE 1ksw

Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP


Overview

The direct substrates of one protein kinase in a cell can be identified by mutation of the ATP binding pocket to allow an unnatural ATP analog to be accepted exclusively by the engineered kinase. Here, we present structural and functional assessment of peptide specificity of mutant protein kinases with unnatural ATP analogs. The crystal structure (2.8 A resolution) of c-Src (T338G) with N(6)-(benzyl) ADP bound shows that the creation of a unique nucleotide binding pocket does not alter the phospho-acceptor binding site of the kinase. A panel of optimal peptide substrates of defined sequence, as well as a degenerate peptide library, was utilized to assess the phospho-acceptor specificity of the engineered "traceable" kinases. The specificity profiles for the mutant kinases were found to be identical to those of their wild-type counterparts.

About this Structure

1KSW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme., Witucki LA, Huang X, Shah K, Liu Y, Kyin S, Eck MJ, Shokat KM, Chem Biol. 2002 Jan;9(1):25-33. PMID:11841936 Page seeded by OCA on Fri May 2 23:08:01 2008

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