This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4oah

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:13, 25 January 2023

Crystal structure of the cytosolic domain of mouse MiD51 H201A mutant

Structural highlights

4oah is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MID51_MOUSE Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins.

Publication Abstract from PubMed

Mitochondrial fission requires recruitment of dynamin-related protein 1 (Drp1) to the mitochondrial surface and activation of its GTP-dependent scission function. The Drp1 receptors MiD49 and MiD51 recruit Drp1 to facilitate mitochondrial fission, but their mechanism of action is poorly understood. Using X-ray crystallography, we demonstrate that MiD51 contains a nucleotidyl transferase domain that binds ADP with high affinity. MiD51 recruits Drp1 via a surface loop that functions independently of ADP binding. However, in the absence of nucleotide binding, the recruited Drp1 cannot be activated for fission. Purified MiD51 strongly inhibits Drp1 assembly and GTP hydrolysis in the absence of ADP. Addition of ADP relieves this inhibition and promotes Drp1 assembly into spirals with enhanced GTP hydrolysis. Our results reveal ADP as an essential cofactor for MiD51 during mitochondrial fission.

The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor.,Loson OC, Liu R, Rome ME, Meng S, Kaiser JT, Shan SO, Chan DC Structure. 2014 Mar 4;22(3):367-77. doi: 10.1016/j.str.2014.01.001. Epub 2014 Feb, 6. PMID:24508339^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Loson OC, Liu R, Rome ME, Meng S, Kaiser JT, Shan SO, Chan DC. The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor. Structure. 2014 Mar 4;22(3):367-77. doi: 10.1016/j.str.2014.01.001. Epub 2014 Feb, 6. PMID:24508339 doi:http://dx.doi.org/10.1016/j.str.2014.01.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4oah"

Categories: Large Structures | Mus musculus | Chan DC | Kaiser JT | Loson OC

@@ Line 1: / Line 1: @@
 ==Crystal structure of the cytosolic domain of mouse MiD51 H201A mutant==
-<StructureSection load='4oah' size='340' side='right' caption='[[4oah]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='4oah' size='340' side='right'caption='[[4oah]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4oah]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OAH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4oah]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OAH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4oaf|4oaf]], [[4oag|4oag]], [[4oai|4oai]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oah OCA], [https://pdbe.org/4oah PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oah RCSB], [https://www.ebi.ac.uk/pdbsum/4oah PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oah ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mid51, MiD51 (aka SMCR7L), Mief1, Smcr7l ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oah OCA], [http://pdbe.org/4oah PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oah RCSB], [http://www.ebi.ac.uk/pdbsum/4oah PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oah ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MID51_MOUSE MID51_MOUSE]] Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins.
+[https://www.uniprot.org/uniprot/MID51_MOUSE MID51_MOUSE] Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Chan, D C]]
+[[Category: Mus musculus]]
-[[Category: Kaiser, J T]]
+[[Category: Chan DC]]
-[[Category: Loson, O C]]
+[[Category: Kaiser JT]]
-[[Category: Nucleotidyl transferase fold]]
+[[Category: Loson OC]]
-[[Category: Transferase]]

4oah

From Proteopedia

Revision as of 07:13, 25 January 2023

Crystal structure of the cytosolic domain of mouse MiD51 H201A mutant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox