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| | ==Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with bound ManNAc== | | ==Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with bound ManNAc== |
| - | <StructureSection load='4m7f' size='340' side='right' caption='[[4m7f]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='4m7f' size='340' side='right'caption='[[4m7f]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4m7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4m7f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M7F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BM3:2-(ACETYLAMINO)-2-DEOXY-ALPHA-D-MANNOPYRANOSE'>BM3</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BM3:2-(ACETYLAMINO)-2-DEOXY-ALPHA-D-MANNOPYRANOSE'>BM3</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m7h|4m7h]], [[1jc9|1jc9]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7f OCA], [https://pdbe.org/4m7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m7f RCSB], [https://www.ebi.ac.uk/pdbsum/4m7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7f ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FIBCD1, UNQ701/PRO1346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7f OCA], [http://pdbe.org/4m7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m7f RCSB], [http://www.ebi.ac.uk/pdbsum/4m7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7f ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FBCD1_HUMAN FBCD1_HUMAN]] Acetyl group-binding receptor which shows a high-affinity and calcium-dependent binding to acetylated structures such as chitin, some N-acetylated carbohydrates, and amino acids, but not to their non-acetylated counterparts. Can facilitate the endocytosis of acetylated components.<ref>PMID:19710473</ref> <ref>PMID:19892701</ref> | + | [https://www.uniprot.org/uniprot/FBCD1_HUMAN FBCD1_HUMAN] Acetyl group-binding receptor which shows a high-affinity and calcium-dependent binding to acetylated structures such as chitin, some N-acetylated carbohydrates, and amino acids, but not to their non-acetylated counterparts. Can facilitate the endocytosis of acetylated components.<ref>PMID:19710473</ref> <ref>PMID:19892701</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Greenhough, T J]] | + | [[Category: Large Structures]] |
| - | [[Category: Holmskov, U]] | + | [[Category: Greenhough TJ]] |
| - | [[Category: Shrive, A K]] | + | [[Category: Holmskov U]] |
| - | [[Category: Fibrinogen-like domain]] | + | [[Category: Shrive AK]] |
| - | [[Category: N-acetyl-binding protein]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
FBCD1_HUMAN Acetyl group-binding receptor which shows a high-affinity and calcium-dependent binding to acetylated structures such as chitin, some N-acetylated carbohydrates, and amino acids, but not to their non-acetylated counterparts. Can facilitate the endocytosis of acetylated components.[1] [2]
Publication Abstract from PubMed
The high-resolution crystal structures of a recombinant fragment of the C-terminal fibrinogen-like recognition domain of FIBCD1, a vertebrate receptor that binds chitin, have been determined. The overall tetrameric structure shows similarity in structure and aggregation to the horseshoe crab innate immune protein tachylectin 5A. The high affinity ligand N-acetyl mannosamine binds in the S1 site, predominantly via the acetyl group with the oxygen and acetamide nitrogen hydrogen-bonded to the protein and the methyl group inserted into a hydrophobic pocket. The binding of the ManNAc pyranose ring differs markedly between the two independent subunits but in all structures the binding of the N-acetyl group is conserved. In the native structure, a crystal contact results in one of the independent protomers binding the first GlcNAc of the Asn340 N-linked glycan on the other independent protomer. In the ligand bound structure this GlcNAc is replaced by the higher affinity ligand ManNAc. In addition, a sulphate ion has been modelled into the electron density at a location similar to the S3 binding site in L-ficolin, while in the native structure an acetate ion has been placed in the S1 N-acetyl binding site and a sulphate ion has been placed adjacent to this site. These ion binding sites are ideally placed to receive the N-acetyl and sulphate groups of sulphated GalNAc residues of glycosaminoglycans such as chondroitin and dermatan sulphate. Together, these structures give insight into important determinants of ligand selectivity, demonstrating versatility in recognition and binding whilst maintaining conservation in N-acetyl and calcium binding.
Crystal structure of the tetrameric fibrinogen-like recognition domain of Fibrinogen C domain containing 1 (FIBCD1).,Shrive AK, Moeller JB, Burns I, Paterson JM, Shaw AJ, Schlosser A, Sorensen GL, Greenhough TJ, Holmskov U J Biol Chem. 2013 Dec 19. PMID:24293368[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schlosser A, Thomsen T, Moeller JB, Nielsen O, Tornoe I, Mollenhauer J, Moestrup SK, Holmskov U. Characterization of FIBCD1 as an acetyl group-binding receptor that binds chitin. J Immunol. 2009 Sep 15;183(6):3800-9. doi: 10.4049/jimmunol.0901526. Epub 2009, Aug 26. PMID:19710473 doi:http://dx.doi.org/10.4049/jimmunol.0901526
- ↑ Thomsen T, Moeller JB, Schlosser A, Sorensen GL, Moestrup SK, Palaniyar N, Wallis R, Mollenhauer J, Holmskov U. The recognition unit of FIBCD1 organizes into a noncovalently linked tetrameric structure and uses a hydrophobic funnel (S1) for acetyl group recognition. J Biol Chem. 2010 Jan 8;285(2):1229-38. doi: 10.1074/jbc.M109.061523. Epub 2009, Nov 5. PMID:19892701 doi:http://dx.doi.org/10.1074/jbc.M109.061523
- ↑ Shrive AK, Moeller JB, Burns I, Paterson JM, Shaw AJ, Schlosser A, Sorensen GL, Greenhough TJ, Holmskov U. Crystal structure of the tetrameric fibrinogen-like recognition domain of Fibrinogen C domain containing 1 (FIBCD1). J Biol Chem. 2013 Dec 19. PMID:24293368 doi:http://dx.doi.org/10.1074/jbc.M113.520577
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