3zif

From Proteopedia

(Difference between revisions)

Revision as of 22:08, 15 November 2017

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Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly

Structural highlights

3zif is a 18 chain structure with sequence from Bovine adenovirus 3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Warning: this is a large structure, and loading might take a long time or not happen at all.

Function

[CAPSH_ADEB3] Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus (By similarity).

Publication Abstract from PubMed

Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level.

Cryo-EM structures of two bovine adenovirus type 3 intermediates.,Cheng L, Huang X, Li X, Xiong W, Sun W, Yang C, Zhang K, Wang Y, Liu H, Huang X, Ji G, Sun F, Zheng C, Zhu P Virology. 2014 Feb;450-451:174-81. doi: 10.1016/j.virol.2013.12.012. Epub 2014, Jan 3. PMID:24503080^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheng L, Huang X, Li X, Xiong W, Sun W, Yang C, Zhang K, Wang Y, Liu H, Huang X, Ji G, Sun F, Zheng C, Zhu P. Cryo-EM structures of two bovine adenovirus type 3 intermediates. Virology. 2014 Feb;450-451:174-81. doi: 10.1016/j.virol.2013.12.012. Epub 2014, Jan 3. PMID:24503080 doi:http://dx.doi.org/10.1016/j.virol.2013.12.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zif"

@@ Line 3: / Line 3: @@
 <StructureSection load='3zif' size='340' side='right' caption='[[3zif]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zif]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeb3 Adeb3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZIF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zif]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_adenovirus_3 Bovine adenovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZIF FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zif OCA], [http://pdbe.org/3zif PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zif RCSB], [http://www.ebi.ac.uk/pdbsum/3zif PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zif ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Adeb3]]
+[[Category: Bovine adenovirus 3]]
 [[Category: Cheng, L]]
 [[Category: Huang, X]]

3zif

From Proteopedia

Revision as of 22:08, 15 November 2017

Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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