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3ryd

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Revision as of 09:58, 16 September 2020

Crystal structure of Ca bound IMPase family protein from Staphylococcus aureus

Structural highlights

3ryd is a 2 chain structure with sequence from Staas. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	3qmf, 2p3n
Gene:	SAS2203 (STAAS)
Activity:	Inositol-phosphate phosphatase, with EC number 3.1.3.25
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Inositol monophosphatase (IMPase) family of proteins are Mg(2+) activated Li(+) inhibited class of ubiquitous enzymes with promiscuous substrate specificity. Herein, the molecular basis of IMPase substrate specificity is delineated by comparative crystal structural analysis of a Staphylococcal dual specific IMPase/NADP(H) phosphatase (SaIMPase - I) with other IMPases of different substrate compatibility, empowered by in silico docking and Escherichiacoli SuhB mutagenesis analysis. Unlike its eubacterial and eukaryotic NADP(H) non-hydrolyzing counterparts, the composite structure of SaIMPase - I active site pocket exhibits high structural resemblance with archaeal NADP(H) hydrolyzing dual specific IMPase/FBPase. The large and shallow SaIMPase - I active site cleft efficiently accommodate large incoming substrates like NADP(H), and therefore, justifies the eminent NADP(H) phosphatase activity of SaIMPase - I. Compared to other NADP(H) non-hydrolyzing IMPases, the profound difference in active site topology as well as the unique NADP(H) recognition capability of SaIMPase - I stems from the differential length and orientation of a distant helix alpha4 (in human and bovine alpha5) and its preceding loop. We identified the length of alpha4 and its preceding loop as the most crucial factor that regulates IMPase substrate specificity by employing a size exclusion mechanism. Hence, in SaIMPase - I, the substrate promiscuity is a gain of function by trimming the length of alpha4 and its preceding loop, compared to other NADP(H) non-hydrolyzing IMPases. This study thus provides a biochemical - structural framework revealing the length and orientation of alpha4 and its preceding loop as the predisposing factor for the determination of IMPase substrate specificity.

Crystal structure of Staphylococcal dual specific inositol monophosphatase/NADP(H) phosphatase (SAS2203) delineates the molecular basis of substrate specificity.,Bhattacharyya S, Dutta D, Saha B, Ghosh AK, Das AK Biochimie. 2011 Dec 16. PMID:22197784^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhattacharyya S, Dutta D, Saha B, Ghosh AK, Das AK. Crystal structure of Staphylococcal dual specific inositol monophosphatase/NADP(H) phosphatase (SAS2203) delineates the molecular basis of substrate specificity. Biochimie. 2011 Dec 16. PMID:22197784 doi:10.1016/j.biochi.2011.12.007

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ryd"

@@ Line 1: / Line 1: @@
-==Crystal strucutre of Ca bound IMPase family protein from Staphylococcus aureus==
+==Crystal structure of Ca bound IMPase family protein from Staphylococcus aureus==
-<StructureSection load='3ryd' size='340' side='right' caption='[[3ryd]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
+<StructureSection load='3ryd' size='340' side='right'caption='[[3ryd]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ryd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staas Staas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RYD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ryd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staas Staas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RYD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3RYD FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qmf|3qmf]], [[2p3n|2p3n]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAS2203 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=282459 STAAS])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-phosphate_phosphatase Inositol-phosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.25 3.1.3.25] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ryd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryd OCA], [http://pdbe.org/3ryd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ryd RCSB], [http://www.ebi.ac.uk/pdbsum/3ryd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ryd ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ryd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryd OCA], [http://pdbe.org/3ryd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ryd RCSB], [http://www.ebi.ac.uk/pdbsum/3ryd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ryd ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Inositol Monophosphatase|Inositol Monophosphatase]]
+*[[Inositol monophosphatase 3D structures|Inositol monophosphatase 3D structures]]
 == References ==
 <references/>
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Inositol-phosphate phosphatase]]
+[[Category: Large Structures]]
 [[Category: Staas]]
 [[Category: Bhattacharyya, S]]

3ryd

From Proteopedia

Revision as of 09:58, 16 September 2020

Crystal structure of Ca bound IMPase family protein from Staphylococcus aureus

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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