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4gyv

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Revision as of 14:29, 22 December 2016

Crystal structure of the DH domain of FARP2

Structural highlights

4gyv is a 9 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	4gzu
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FARP2_MOUSE] Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton.^[1] ^[2]

Publication Abstract from PubMed

FARP2 is a Dbl-family guanine nucleotide exchange factor (GEF) that contains a 4.1, ezrin, radixin and moesin (FERM) domain, a Dbl-homology (DH) domain and two pleckstrin homology (PH) domains. FARP2 activates Rac1 or Cdc42 in response to upstream signals, thereby regulating processes such as neuronal axon guidance and bone homeostasis. How the GEF activity of FARP2 is regulated remained poorly understood. We have determined the crystal structures of the catalytic DH domain and the DH-PH-PH domains of FARP2. The structures reveal an auto-inhibited conformation in which the GEF substrate-binding site is blocked collectively by the last helix in the DH domain and the two PH domains. This conformation is stabilized by multiple interactions among the domains and two well-structured inter-domain linkers. Our cell-based activity assays confirm the suppression of the FARP2 GEF activity by these auto-inhibitory elements.

Structural Basis for Autoinhibition of the Guanine Nucleotide Exchange Factor FARP2.,He X, Kuo YC, Rosche TJ, Zhang X Structure. 2013 Mar 5;21(3):355-64. doi: 10.1016/j.str.2013.01.001. Epub 2013 Jan, 31. PMID:23375260^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kubo T, Yamashita T, Yamaguchi A, Sumimoto H, Hosokawa K, Tohyama M. A novel FERM domain including guanine nucleotide exchange factor is involved in Rac signaling and regulates neurite remodeling. J Neurosci. 2002 Oct 1;22(19):8504-13. PMID:12351724
↑ Toyofuku T, Yoshida J, Sugimoto T, Zhang H, Kumanogoh A, Hori M, Kikutani H. FARP2 triggers signals for Sema3A-mediated axonal repulsion. Nat Neurosci. 2005 Dec;8(12):1712-9. Epub 2005 Nov 13. PMID:16286926 doi:nn1596
↑ He X, Kuo YC, Rosche TJ, Zhang X. Structural Basis for Autoinhibition of the Guanine Nucleotide Exchange Factor FARP2. Structure. 2013 Mar 5;21(3):355-64. doi: 10.1016/j.str.2013.01.001. Epub 2013 Jan, 31. PMID:23375260 doi:http://dx.doi.org/10.1016/j.str.2013.01.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4gyv"

@@ Line 1: / Line 1: @@
-==Crystal strucure of the DH domain of FARP2==
+==Crystal structure of the DH domain of FARP2==
 <StructureSection load='4gyv' size='340' side='right' caption='[[4gyv]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4gyv]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GYV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4gyv]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GYV FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gzu|4gzu]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Farp2, Kiaa0793 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyv OCA], [http://pdbe.org/4gyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gyv RCSB], [http://www.ebi.ac.uk/pdbsum/4gyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gyv ProSAT]</span></td></tr>
 </table>
@@ Line 24: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
 [[Category: He, X]]
 [[Category: Zhang, X]]

4gyv

From Proteopedia

Revision as of 14:29, 22 December 2016

Crystal structure of the DH domain of FARP2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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