1l0b
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1l0b.gif|left|200px]] | [[Image:1l0b.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1l0b", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | | | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | + | --> | |
| - | + | {{STRUCTURE_1l0b| PDB=1l0b | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal Structure of rat Brca1 tandem-BRCT region''' | '''Crystal Structure of rat Brca1 tandem-BRCT region''' | ||
| Line 31: | Line 28: | ||
[[Category: Livingston, D M.]] | [[Category: Livingston, D M.]] | ||
[[Category: Pavletich, N P.]] | [[Category: Pavletich, N P.]] | ||
| - | [[Category: | + | [[Category: Tandem-brct]] |
| - | [[Category: | + | [[Category: Three-helix bundle]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:23:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:23, 2 May 2008
Crystal Structure of rat Brca1 tandem-BRCT region
Overview
Brca1 C-terminal (BRCT) domains are a common protein-protein interaction motif in proteins involved in the DNA damage response and DNA repair. The DNA-damage response protein 53BP1 has two BRCT domains that bind to the DNA-binding domain of p53. The 53BP1 tandem-BRCT region is homologous to the tandem-BRCT region of Brca1, which is involved in double-strand break repair and homologous recombination and which binds BACH1, a member of the DEAH helicase family. Here we report the structures of a human 53BP1-p53 complex and of the rat Brca1 BRCT repeats. The 53BP1-p53 structure shows that the two BRCT repeats are arranged tandemly and pack extensively through an interface that also involves the inter-repeat linker. The first BRCT repeat and the linker together bind p53 on a region that overlaps with the DNA-binding surface of p53 and involves p53 residues that are mutated in cancer and are important for DNA binding. Comparison with the structure of the tandem-BRCT region of Brca1 shows a remarkable conservation of the repeat arrangement and of the inter-BRCT repeat interface. Analysis of human BRCA1 tumor-derived mutations and conservation identifies a potential protein-binding site that we show through mutagenesis is involved in BACH1 binding. The BACH1-binding region of Brca1 consists of a unique insertion in the first BRCT repeat and the inter-repeat linker and is analogous to the region of 53BP1 that binds p53.
About this Structure
1L0B is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure., Joo WS, Jeffrey PD, Cantor SB, Finnin MS, Livingston DM, Pavletich NP, Genes Dev. 2002 Mar 1;16(5):583-93. PMID:11877378 Page seeded by OCA on Fri May 2 23:23:42 2008
