1l0z

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[[Image:1l0z.gif|left|200px]]
[[Image:1l0z.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1l0z |SIZE=350|CAPTION= <scene name='initialview01'>1l0z</scene>, resolution 1.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1l0z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=XE:XENON'>XE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1l0z| PDB=1l0z | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l0z OCA], [http://www.ebi.ac.uk/pdbsum/1l0z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l0z RCSB]</span>
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}}
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'''THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL'''
'''THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL'''
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[[Category: Panjikar, S.]]
[[Category: Panjikar, S.]]
[[Category: Tucker, P A.]]
[[Category: Tucker, P A.]]
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[[Category: bromide]]
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[[Category: Bromide]]
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[[Category: ppe]]
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[[Category: Ppe]]
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[[Category: xenon]]
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[[Category: Xenon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:25:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:56:29 2008''
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Revision as of 20:25, 2 May 2008

Image:1l0z.gif

Template:STRUCTURE 1l0z

THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL


Overview

Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.

About this Structure

1L0Z is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Xenon derivatization of halide-soaked protein crystals., Panjikar S, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1413-20. Epub 2002, Aug 23. PMID:12198297 Page seeded by OCA on Fri May 2 23:25:05 2008

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