1l1n
From Proteopedia
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'''POLIOVIRUS 3C PROTEINASE''' | '''POLIOVIRUS 3C PROTEINASE''' | ||
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[[Category: Plotch, S.]] | [[Category: Plotch, S.]] | ||
[[Category: Sia, S.]] | [[Category: Sia, S.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:26:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:26, 2 May 2008
POLIOVIRUS 3C PROTEINASE
Overview
The X-ray crystallographic structure of the recombinant poliovirus 3C gene product (Mahoney strain) has been determined by single isomorphous replacement and non-crystallographic symmetry averaging and refined at 2.1 A resolution. Poliovirus 3C is comprised of two six-stranded antiparallel beta-barrel domains and is structurally similar to the chymotrypsin-like serine proteinases. The shallow active site cleft is located at the junction of the two beta-barrel domains and contains a His40, Glu71, Cys147 catalytic triad. The polypeptide loop preceding Cys147 is flexible and likely undergoes a conformational change upon substrate binding. The specificity pockets for poliovirus 3C are well-defined and modeling studies account for the known substrate specificity of this proteinase. Poliovirus 3C also participates in the formation of the viral replicative initiation complex where it specifically recognizes and binds the RNA stem-loop structure in the 5' non-translated region of its own genome. The RNA recognition site of 3C is located on the opposite side of the molecule in relation to its proteolytic active site and is centered about the conserved KFRDIR sequence of the domain linker. The recognition site is well-defined and also includes residues from the amino and carboxy-terminal helices. The two molecules in the asymmetric unit are related by an approximate 2-fold, non-crystallographic symmetry and form an intermolecular antiparallel beta-sheet at their interface.
About this Structure
1L1N is a Single protein structure of sequence from Human poliovirus 1. Full crystallographic information is available from OCA.
Reference
Refined X-ray crystallographic structure of the poliovirus 3C gene product., Mosimann SC, Cherney MM, Sia S, Plotch S, James MN, J Mol Biol. 1997 Nov 14;273(5):1032-47. PMID:9367789 Page seeded by OCA on Fri May 2 23:26:15 2008
