1l1w
From Proteopedia
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'''NMR structure of a SRP19 binding domain in human SRP RNA''' | '''NMR structure of a SRP19 binding domain in human SRP RNA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1W OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of a SRP19 binding domain in human SRP RNA., Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G, J Biochem. 2002 Aug;132(2):177-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12153712 12153712] | Solution structure of a SRP19 binding domain in human SRP RNA., Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G, J Biochem. 2002 Aug;132(2):177-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12153712 12153712] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Kawai, G.]] | [[Category: Kawai, G.]] | ||
[[Category: Morita, S.]] | [[Category: Morita, S.]] | ||
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[[Category: Sakamoto, T.]] | [[Category: Sakamoto, T.]] | ||
[[Category: Tabata, K.]] | [[Category: Tabata, K.]] | ||
| - | [[Category: | + | [[Category: Gnrr]] |
| - | [[Category: | + | [[Category: Mismatch]] |
| - | [[Category: | + | [[Category: Rna hairpin]] |
| - | [[Category: | + | [[Category: Tetraloop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:26:58 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:26, 2 May 2008
NMR structure of a SRP19 binding domain in human SRP RNA
Overview
Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of a SRP19 binding domain in human SRP RNA., Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G, J Biochem. 2002 Aug;132(2):177-82. PMID:12153712 Page seeded by OCA on Fri May 2 23:26:58 2008
Categories: Kawai, G. | Morita, S. | Nakamura, K. | Sakamoto, T. | Tabata, K. | Gnrr | Mismatch | Rna hairpin | Tetraloop
